Figure 1. Overall Scheme.

Glycosyltransferase activity assays were performed in miniaturized microwells using cell lysates as source of enzyme activity. Such studies performed using cell lines ‘predict’ activities that may be altered during cancer. These predictions are tested using human patient tumor and normal tissue samples. Glycan structures that were predicted to be altered based on glycoT activity assays are ‘validated’ using lectin and antibody based histochemical analysis of tissue microarrays and sections.