FIG 10.

In vitro expression of caAkt alters gene expression in myotubes. (A) Western blot analysis of whole-cell lysate following selective passaging to confirm overexpression of caAkt in retrovirus-transformed C₂C₁₂ myotubes. (B) Representative images of myotube formation in control and caAkt-transformed C₂C₁₂ cells following 5 days of differentiation. (C and D) qPCR analysis of mRNA from C₂C₁₂ myotubes 5 days following Ad-FOXO1 AAA infection relative to Ad-GFP control (=100%) and normalized to control H3f3a for genes of transcriptional regulators (C) or oxidative phosphorylation (OXPHOS) and fatty acid oxidation (FAO) (D). (E) qPCR for human FOXO1 cDNA as encoded by the Ad-FOXO1 AAA adenovirus. n = 5 to 6. n.d., not detected. Data shown as mean ± SEM. *, P < 0.05 versus Ad-GFP-infected control cells.