FIG 4.

Impaired energy signaling and mitochondrial enzymatic activities in hearts of 6- and 18-week-old caAkt mice. (A) Western blot analysis of whole-heart protein extract from wild-type (WT) and caAkt mice at 6 weeks of age (left). Quantification of Western blot analysis for phosphorylated AMPKα at Thr172 (p-T¹⁷²) to total AMPKα (n = 6). (B) Aconitase enzymatic activity in mitochondrial and cytosolic fractions of cardiac tissue from 6-week-old WT and caAkt mice. (C) Carnitine palmitoyltransferase (CPT) enzymatic activities in isolated mitochondria from hearts of 6-week-old WT and caAkt mice (n = 4 to 6). (D) Hydroxyacyl-CoA dehydrogenase (HADH) in hearts from 6- and 18-week-old WT and caAkt mice (n = 4 to 6). (E) Citrate synthase (CS) enzymatic activities in WT and caAkt hearts (n = 4 to 6). Data shown as mean ± SEM. *, P < 0.05 versus age-matched WT.