FIG 5.

Short-term activation of Akt increased glycolysis and reduced FAO in the heart, independently of hypertrophy. (A) Representative immunoblots and ratios of phosphorylated Akt at Ser473 (p-S⁴⁷³) to total Akt in hearts from IND-Akt mice withdrawn from doxycycline (DOX) (n = 3). (B) Heart weight-to-body weight ratio (HW/BW) changes with time zero (n = 3 or 4) and 3 (n = 3), 7 (n = 3 or 4), 10 (n = 9), 14 (n = 3), 21 (n = 6), and 42 (n = 4 or 8) days in control mice (Con) and IND-Akt mice, respectively. (C) Dry heart weight (DHW)-to-body weight ratios (DHW/BW) in control and IND-Akt mice withdrawn from DOX for 14 days and treated daily with rapamycin (Rap) were obtained after isolated working heart perfusions (n = 9). (D to F) Glycolysis (n = 4) (D), glucose oxidation (n = 4) (E), and palmitate oxidation (n = 5) (F) in isolated working hearts from control and IND-Akt mice withdrawn from DOX for 14 days and treated daily with rapamycin (Rap). Data shown as mean ± SEM. *, P < 0.05 versus induction and treatment-matched control.