FIG 8.

Short-term transgenic activation of Akt is associated with inhibition of FOXO and AMPK. (A) Representative Western blot analysis of whole-heart extract from Con or tON-Akt mice following 10 days of DOX treatment. (B to G) Quantification of Western blot analysis for ratios of phosphorylated to total protein or total protein to loading control (GAPDH): FOXO1 at Thr24 (p-T²⁴), FOXO3 at Ser318,321 (p-S^318,321), AMPKα at Thr172 (p-T¹⁷²) to total protein and total FOXO1, FOXO3, and AMPKα to GAPDH (n = 6). Data shown as mean ± SEM normalized to control (=1.0). *, P < 0.05 versus control.