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. 2015 Feb 3;35(5):831–846. doi: 10.1128/MCB.01109-14

FIG 9.

FIG 9

FOXO transcription factors may regulate Akt-mediated modulation of mitochondrial gene expression. (A) qPCR quantification of DNA promoter occupancy following chromatin immunoprecipitation (ChIP) in control and 8-week-old caAkt mouse hearts for antibody to FOXO1 (Ab-FOXO1) or positive-control RNA polymerase II (Ab-RPB1) (n = 3). Data shown as mean ± SEM. *, P < 0.05 versus WT. Candidate response elements are defined in Table S5 in the supplemental material. (B) Representative pictures of cardiomyocytes isolated from IND-Akt (14-day) hearts after in vivo adenoviral injection of GFP and constitutively active FOXO1 (Ad-FOXO1 AAA). FITC, fluorescein isothiocyanate. (C) qPCR from GFP-negative and GFP-positive cells; arbitrary units normalized to GFP-negative Con cells (=1.0) (n = 2 to 4 independent harvests of 10 cells per sample). (D) qPCR from primary neonatal ventricular cardiomyocytes (NRVCMs) following Foxo1 and/or Foxo3 siRNA knockdown relative to scrambled siRNA control (=100%) and normalized to GAPDH (n = 6 to 9). Data shown as mean ± SEM. *, P < 0.05 versus Con.