Table 1.
Details of the various fly strains used in this study
| Genotype | Description | Protein level (%WT) | Reference |
|---|---|---|---|
| w1118 (Abbreviation: WT) | White-eyed | ||
| yw;P[GMR:Gal4, UAS:w-RNAi, y]/Cyo; P[UAS:IP3R-RNAi, w] (Abbreviation: IP3R-RNAi) | White-eyed transgenic fly with reduced expression level of IP3R | ∼15% IP3R (Fig. 1A) | P[UAS-IP3R-RNAi] transgenic line no. 6486 from VDRC (Dietzl et al., 2007) |
| Stable line | yw;P[GMR:Gal4, UAS:w-RNAi, y]/CyO, a gift from A. Huber | ||
| Progenies of the cross: w;P[UAS:GCaMP6f]; X | Transgenic fly expressing the Ca2+ indicator GCaMP6f on a WT background | P[UAS-GCaMP6f] was obtained from Bloomington stock center #42747 (Chen et al., 2013) | |
| yw;P[GMR:Gal4, UAS:w-RNAi, y] (Abbreviation: wt/GCaMP6f) | Cross progeny | ||
| Progenies of the cross: yw;P[GMR:Gal4, UAS:w-RNAi, y]/cyo; P[UAS:IP3R-RNAi, w]; X | White-eyed transgenic fly expressing GCaMP6f on background of reduced concentration of IP3R | ||
| P[UAS:GCaMP6f] (Abbreviation: GCaMP6f;IP3R-RNAi) | Cross progeny | ||
| Progenies of the cross: norpAH43;bw;st | White-eyed transgenic fly expressing reduced concentration of IP3R on white-eyed PLCβ mutant background | ∼79% PLCβ, ∼15% IP3R | |
| X | |||
| yw;P[y+,GMR-Gal4,UAS-]/CyO; P[UAS-RNAi-IP3R,w] (Abbreviation: norpAH43;IP3R-RNAi) | Cross progeny | ||
| norpAH43;bw;st (Abbreviation: norpAH43) | White-eyed PLCβ mutant with Ser347Asn and Thr1007Ser mutations | A gift from W.L. Pak (Yoon et al., 2004) |