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Intracellular solution for whole cell recordings
| Solution name | D-Gluconic acid potassium salt (KGlu, mm) | MgSO4 (mm) | TES (mm) | MgATP (mm) | NaGTP (mm) | EGTA | NAD (mm) | CaCl2 (mm) |
|---|---|---|---|---|---|---|---|---|
| KGlu (0 mm [Ca2+]i) | 140 | 2 | 10 | 4 | 0.4 | — | 1 | — |
| KGlu + 1 mM EGTA (0 mm [Ca2+]i + EGTA) | 140 | 2 | 10 | 4 | 0.4 | 1.0 | 1 | — |
| KGlu + 1 mM EGTA + 0.45 mM [Ca2+]i (∼300 nM free Ca2+) | 140 | 2 | 10 | 4 | 0.4 | 1.0 | 1 | 0.45 |
