Figure 6.

Partial loss and disorganization of dopaminergic neurons in the PT of the ventral diencephalon and downregulation of critical genes involved in specification, differentiation, and development or maintenance of dopaminergic neurons in per1b mutant zebrafish. A, B, Dopaminergic neurons in the PT of the ventral diencephalon of 5 dpf larvae (anterior to left), stained by anti-TH antibody. Scale bar, 50 μm. C, Numbers of positive TH neurons in per1b mutants and wild types, quantified with ImageJ cell counter function in double-blind scoring. Student's t test. D–I, Downregulation of signaling proteins wnt1 (D), wnt3a (E), and wnt5a (F) and of transcription factors otpa (G), otpb (H), and manf (I) involved in specification, differentiation, development, or maintenance of the dopaminergic neurons under DD conditions. Experiments were performed in triplicate, with three biological samples for each experiment. Data were analyzed using the Student's t test. All error bars are ±SEM. Numbers on the right side of the star indicate p values.