Figure 7.

Continual loss of dopaminergic neurons in the PT of the ventral diencephalon from 60–84 hpf in per1b mutant zebrafish. A, C, E, Dopaminergic neurons in the wild-type PT. B, D, F, Dopaminergic neurons in the per1b mutant PT. Scale bar, 20 μm. G–I, The numbers of dopaminergic neurons in wild-type and per1b mutant larvae, determined with ImageJ in a double-blind manner. n = 12. Data were calculated in three independent experiments. Student's t test was used in the statistical analysis. Error bars are ±SEM. Numbers on the right side of the star indicate p values.