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. 2015 Feb 11;35(6):2803–2816. doi: 10.1523/JNEUROSCI.4557-14.2015

Figure 2.

Figure 2.

LGC-35 is a GABA-gated cation channel. A, Whole-cell currents recorded from X. laevis oocytes expressing LGC-35 in response to 1 mm: glutamate, serotonin (5-HT), choline, acetylcholine, glycine, and GABA. Black bar represents 10 s application of ligand. Only GABA application evoked whole-cell currents in LGC-35-expressing oocytes. B, Representative traces of LGC-35 dose–response experiments. Black bar represents 1–1000 μm GABA application for 30 s. C, Dose–response curve for LGC-35-expressing oocytes. Oocytes expressing LGC-35 were voltage-clamped at −60 mV, and GABA was bath-applied in series (1–1000 μm) for 5 s. Points indicate mean current value normalized to maximum value. For LGC-35 receptors, EC50 = 15.04 ± 1.04 μm and Hill coefficient = 1.9 (n = 26). Error bars indicate SEM. D, Current–voltage relations of LGC-35-expressing oocytes. I-V curves determined in control Ringer's solution (Erev = −10.86 ± 1.15 mV, n = 12, black), Cl− (Erev = −12.94 ± 2.79 mV, n = 8, blue), and Na+ Free Ringer (Erev = −76.85 ± 0.65 mV, n = 7, red), K+ Ringer's (Erev = 6.35 ± 1.26 mV, n = 7, green). Each point indicates the mean ± SD.