Fig. 2.

NS2 interacts with endogenous CHD3 under infection. a NS2 colocalizes with endogenous CHD3 under WD infection. COS-1 cells on coverslips were infected with WD [0.5 multiplicity of infection (MOI)], and at 5 h p.i., the cells were fixed for IFA with antibodies against NS2 and CHD3. b NS2 bound endogenous CHD3 during virus infection. COS-1 cells were infected by the WD or WD-Flag-NS2 viruses at an MOI of 3. At 8 h p.i., cell lysates were subjected to the IP assay with anti-Flag monoclonal antibody. The immunoprecipitated proteins were assayed with indicated antibody. Colocalization was analyzed using Image J and the coefficients confirmed the strong colocalization