Fig. 6.

The NS2–CHD3 interaction modulated the propagation of the influenza A virus. a The WD–NS2(L19S) virus containing weaker NS2–CHD3 interaction exhibited poor propagation. COS-1 cells were infected and the supernatants were collected at the indicated time points for detecting the viral titer. b The knockdown of CHD3 (a) significantly decreased WD viral titers (b). c The knockdown of CHD3 (a) decreased WD–NS2(L19S) viral titers to a small extent (b). COS-1 cells were transfected with si-CHD3 or si-NC and infected with the WD or WD–NS2(L19S) virus (MOI 0.1) at the 24 h post-transfection. At 12, 24 and 36 h p.i., the cell supernatants were collected to measure the viral titer. The results are presented as the mean ± SD (*p < 0.05, **p < 0.01, n = 3)