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. 2015 Feb 11;5:8398. doi: 10.1038/srep08398

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Isothermal titration calorimetry (iTC) studies to evaluate the binding between DNA and pyocyanin. Upper panel: Raw data for the titration of total 200 μl DNA (50 ng/μl) with total ~310 μM pyocyanin. Lower panel: Integrated, dilution-corrected and concentration-normalized titration data of the DNA with pyocyanin. Data were fitted with the ‘one binding site' model using Origin 7.0 data analysis software (MicroCal) with derived thermodynamic parameters including enthalpy (ΔH), entropy (ΔS) and Gibbs free energy (ΔG) at 25°C.