Figure 4. Square wave voltammograms (SWV) of accumulated pyocyanin on modified gold electrodes before (black) and after (red) hybridization with target (a), noncomplementary (b) and single adenine-cytosine mismatch (c).

Pyocyanin was accumulated for 30 min in a solution containing 100 μM of pyocyanin in PBS following by rinsing with 0.5 mL PBS. Measurements were performed in a solution containing 2.5 μM pyocyanin in PBS. Pulse amplitude: 25 mV, Step: 4 mV, Frequency: 10 Hz.