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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 16;194(4):1467–1479. doi: 10.4049/jimmunol.1402807

FIGURE 5.

FIGURE 5

The effects of Irf5 expression in bone marrow-derived and non-bone marrow-derived cells on disease parameters in gld.apoE−/− mice. Irf5+/+.gld.apoE−/− and Irf5−/−.gld.apoE−/− recipients were lethally irradiated and then reconstituted with bone marrow from either Irf5+/+gld.apoE−/− or Irf5−/− gld.apoE−/− donor mice. Mice were placed on western diet for 6-9 weeks and disease parameters were analyzed at 16-19 weeks of age. (A) spleen and (B) cervical lymph node weights: +/+→+/+ (n=8), +/+→−/− (n=5-6), −/− → +/+ (n=9) and −/− →−/− (n=9). (C) Serum anti-nuclear autoantibody (ANA) titers: +/+ → +/+ (n=6), +/+→−/− (n=4), −/− → +/+ (n=6) and −/− → −/− (n=3). (D) Serial aortic root sections were stained with Oil Red O to detect lipid deposition and the aortic root lesion size was quantitated: +/+ → +/+ (n=3), +/+→−/− (n=4), −/− → +/+ (n=4) and −/− → −/− (n=4). (E) Perigonadal and subcutaneous fat pad weights: +/+ → +/+ (n=8), +/+→−/− (n=6), −/− → +/+ (n=9) and −/− → −/− (n=10). (F) Serum triglyceride, cholesterol, non-esterified free fatty acid and phospholipid levels: +/+ → +/+ (n=8-10), +/+→−/− (n=7-10), −/−→ +/+ (n=6-9) and −/− → −/− (n=8-9). Error bars represent the mean and SEM. 2-way ANOVA P values are shown for the effect of IRF5 deficiency in the donor (D) and the effect of IRF5 deficiency in the recipient (R).