The rate equations were solved numerically with re-optimized parameters listed in Appendix H for: (A&B) a presenilin-1 mutation carrier with plaques validated by PET PIB, and (C&D) a non-carrier without plaques. The predicted time course of plasma leucine, APP, C99 and Aβ42 in the brain compartment are shown in A&C. The predicted time course of Aβ42 in the brain, first delay compartment, second delay compartment, and third delay compartment are shown in B&D. The third delay compartment corresponds to the lumber sub-arachnoid space from which CSF was sampled.