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. 2015 Feb 7;21:148–159.

Figure 2.

Figure 2

Overexpression of Kcnj10 in retinal explant culture and rescue of phenotype induced by sh-Kcnj10. A, B: Expression plasmid of Kcnj10 or empty vector and enhanced green fluorescent protein (EGFP)-expressing plasmid was electroporated into the isolated retina at E18, and cultured for the indicated days. A: Proliferation and apoptotic cells were examined with immunostaining of Ki67 or active caspase 3 (aCasp3), respectively. B: Immunostaining patterns of 4',6-diamidino-2-phenylindole (DAPI), EGFP, and glutamine synthetase (GS) are shown. C: Control, CAG-Kcnj10, sh-Kcnj10, or CAG-Kcnj10 + sh-Kcnj10 was introduced into the retina at E18, and cultured for 2 weeks. Then, morphology and differentiation were examined with immunostaining of frozen sections. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.