Figure 3.

Effects of barium chloride for retinal explants. Retinal explants were prepared from isolated mouse retinas at E18, and cultured as indicated days in the presence or absence of barium chloride (Ba²⁺) in the culture medium. Explants were frozen sectioned, and immunostaining was done with indicated antibodies. C: The number of retinal cells, which was counted with 4',6-diamidino-2-phenylindole (DAPI) signals, in each layer. Numbers of cells of the GCL, and the sum of the INL and the ONL are shown. D: The thickness of the INL and the ONL. E: Number of active caspase 3 (Casp3) positive cells is shown. Nuclei were visualized with DAPI staining (gray) in A, F–H. P value; * <0.05, **<0.01 (the Student t test from at least three independent samples).