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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 21;194(4):2022–2031. doi: 10.4049/jimmunol.1402661

Figure 1. Production and validation of CD45 Ab conjugates used for barcoding.

Figure 1

(A) Workflow for the generation of Pd-labeled CD45 antibodies using isothiocyanobenzyl-EDTA. The blue box reflects isothiocyanobenzyl-EDTA, the structure of which is depicted in the blue insert. (B) CD45 antibody retains specificity after Pd-labeling. PBMC were stained with CD45-Pd106 with or without prior incubation with excess unlabeled CD45 antibody and analyzed by mass cytometry. Numbers reflect geometric mean signal intensities. (C) PBMC were stained with one or combinations of up to three different CD45 Abs labeled with the indicated metal ions. Numbers reflect geometric mean signal intensities. The background visible in the ‘unstained’ control for CD45-In115 results from isotopic impurity of In113 used in the staining with CD45-In113.