Figure 3.
Inhibitory effect of antiphospholipid antibodies (aPLs) on scratch assay for endothelial cell migration does not involve effect on cell proliferation. A, Bovine aortic endothelial cells (BAECs) were seeded in 6‐well plates (1×105 cells/well) and grown for 24 hours. Cells were then incubated for 0, 24, and 48 hours in the presence of human IgG from healthy nonautoimmune individuals (NHIgG) (100 μg/mL) or aPLs (100 μg/mL), and the number of the cells/well was counted. There were no difference between cells treated with NHIgG and aPLs. Values are mean±SEM, n=3/group, ns; not significant. B, In scratch assays, BAECs were wounded and treated with PBS vehicle or hydroxyurea (1 mmol/L), vascular endothelial growth factor (VEGF; \(50 ng/mL), or VEGF in the presence of NHIgG (100 μg/mL) or aPLs (100 μg/mL) for 24 hours, and cell migration was evaluated. Values are mean±SEM, n=3/group, *P<0.05 vs no VEGF, †P<0.05 vs NHIgG.