Table 2.
Protection in mice against challenge with 100 MID50 (~6 log10 EID50) of wild type virus A/Mallard/Netherlands/ 12/00(H7N3) following immunization with H7N3 LAIV1.
| Preparation | Survived on day 14 p.i./ total | Mean % of weight loss on day 6 p.i. | Virus isolation from tissues at day 3 pi | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Lungs | Nasal turbinates | Brain | Spleen | |||||||
| No/ total | Virus titers (log10EID50/mL) | No/ total | Virus titers (log10EID50/mL) | No/ total | Virus titers (log10EID50/mL) | No/ total | Virus titers (log10EID50/mL) | |||
| H7N3 LAIV 1000 MID50 | 5/5 | 1 | 0/3 | ≤1.5±0.0 | 0/3 | ≤1.5±0.0 | 0/3 | ≤1.5±0.0 | 0/3 | ≤1.5±0.0 |
| H7N3 LAIV 100 MID50 | 5/5 | 6 | 0/3 | ≤1.5±0.0 | 0/3 | ≤1.5±0.0 | 0/3 | ≤1.5±0.0 | 0/3 | ≤1.5±0.0 |
| PBS | 5/5 | 9 | 3/3 | 5.7 ±1.3 | 3/3 | 4.2 ±0.3 | 0/3 | ≤1.5±0.0 | 0/3 | ≤1.5±0.0 |
1
Challenge was done 21 days after 2nd vaccine dose
2
P=0.049 compared to vaccinated mice
3
P=0.007 compared to vaccinated mice