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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Virology. 2014 Dec 30;476:240–248. doi: 10.1016/j.virol.2014.12.019

Fig. 4.

Fig. 4

gp120-sfGFP can be used to screen gp120 inhibitors by LSC. TZM-bl cells grown overnight in optical imaging microplates were exposed to (A) a range of concentrations of gp120-sfGFP in the presence or absence of (B) 10 μg/mL anti-HIV antibodies (VRC01, 2G12, and 48d) or 10 μg/mL isotype-matched control antibodies (claudin-5 and nectin-1). After 1 h of incubation, media was replaced and nuclei were stained using Hoechst; LSC was then used to identify nuclei and GFP intensities in 10 fields per well with triplicate wells. The SS-sfGFP control was used to set the negative gate to enable detection of specific GFP cell labeling. Results are representative of two independent experiments.