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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 14;194(4):1776–1787. doi: 10.4049/jimmunol.1401684

Figure 1. Effect of VIP treatment.

Figure 1

PCR array of VIP and PBS treated mouse corneas at 3 days p.i. (n=5/group) showed a 13.72 fold down-regulation of HMGB1 after VIP treatment (A). Real time RT-PCR showed significant down-regulation of HMGB1 in VIP treated corneas at 1, 3, 5, and 7 days p.i. (p<0.001, p<0.001, p<0.05 and p<0.01), but no difference between normal, uninfected corneas (n=5/group/time) (B). ELISA analysis showed significantly less HMGB1 protein in the normal, uninfected corneas of VIP compared to PBS treated mice (p<0.01) and at 3 and 5 days p.i. (p<0.001 for both) (C). (n=5/group/time) Data shown in (B) and (C) analyzed using a two-tailed Student’s t-test and shown as the mean ± SEM.