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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 14;194(4):1776–1787. doi: 10.4049/jimmunol.1401684

Figure 4. Real time RT-PCR.

Figure 4

RT-PCR revealed significantly reduced expression of mRNA for (A) IL-1β (p<0.001), (B) MIP-2 (p<0.001), (C) TNF-α (p<0.001), (D) TLR4 (p<0.001), and (E) RAGE (p<0.01) after silencing HMGB1. mRNA expression for (F) SIGIRR and (G) ST2 were significantly elevated after HMGB1 silencing (p<0.01 and 0.05, respectively) (n=5/group/time). Data shown in (A–G) analyzed using a two-tailed Student’s t-test and shown as the mean ± SEM.