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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 14;194(4):1776–1787. doi: 10.4049/jimmunol.1401684

Figure 5. ELISA and MPO assay.

Figure 5

Protein levels for IL-1β (A) were significantly reduced at 5 days p.i. after silencing HMGB1 compared to scrambled control treatment (p<0.01) with no difference seen between groups at 3 days p.i. or in normal, uninfected corneas. Silencing HMGB1 significantly reduced MIP-2 protein levels (B) at 3 and 5 days p.i. compared to scrambled controls (p<0.01 and p=0.05). No difference was detected between groups in normal, uninfected cornea. MPO assay (C) detected fewer PMN in the cornea of siHMGB1 vs scrambled control treated mice (p=0.02). (n=5/group/time for each assay) Data shown in (A-C) analyzed using a two-tailed Student’s t-test and shown as the mean ± SEM.