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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 14;194(4):1776–1787. doi: 10.4049/jimmunol.1401684

Figure 6. RT-PCR and ELISA for inflammatory cell recruitment complex molecules.

Figure 6

(A–E). Significantly less CXCL12 mRNA was detected in the cornea after silencing HMGB1 at 1 day p.i. (p<0.001) compared to scrambled siRNA treatment No significant difference was detected between groups in normal, uninfected cornea or at 3 and 5 days p.i. (A). CXCL12 protein was reduced in the normal (p<0.04) and at 5 days p.i. (p<0.001) cornea after siHMGB1 treatment (B). Expression of CXCR4 was significantly reduced in the cornea of siHMGB1 compared to scrambled control treated mice at 1, 3, and 5 days p.i. (p<0.001, 0.01, and 0.01). No difference was detected between groups in normal, uninfected corneas (C). CXCR4 protein was significantly reduced in the corneas of siHMGB1 vs control treated mice at 5 days p.i. (p<0.01). No difference was detected between groups in normal, uninfected corneas or at 3 days p.i. (D).