Table 2.
Primers and Sequences Used for Standard and Real-time RT-PCR.
| RT-PCR | Primer name | Primer sequence (5’→3’) | Amplicon size (bp) | Source |
|---|---|---|---|---|
| Standard | CaPDPNF1 | AGATGTGGAGGGTGCCAGT | 534 | This study. Designed based on NM_001003220. |
| CaPDPNR1 | AATTCTTCAGCTCTTTAGGGCGAG | |||
| Standard | CaLYVE-1F1 | CTGGTGGTTGTCTGCTTCCAT | 761 | This study. Designed based on XM_003639783. |
| CaLYVE-1R2 | TGCAAAGAAGAGGAGTGCGAG | |||
| Standard | CaProx-1F1 | AATAGCCTCTAAACAGTTTC | 382 | This study. Designed based on XM_853042. |
| CaProx-1R1 | TATCCTCCTGATGTACTTCG | |||
| Standard | CaVEGFR-3F3 | GAGCTCTATGACATCCAGCTGT | 537 | This study. Designed based on XM_538585. |
| CaVEGFR-3R3 | GGCACATTCACCACCAGCTCCAG | |||
| Standard | CaCCL21F1 | TCCACCTCGCGCACTACTC | 414 | This study. Designed based on NM_001005258. |
| CaCCL21R2 | CTCTAGGCTGGTCACTGGG | |||
| Standard | CaGAPDHF1 | ATGGTGAAGGTCGGAGTCAA | 300 | This study. Designed based on NM_001003142. |
| CaGAPDHR1 | GAAGACCCCAGTGGACTCCA | |||
| Standard | FePDPNF1 | ATGTGGAGGGTGCCAGTTCT | 430 | This study. Designed based on obtained ferret sequence. |
| FePDPNR1 | CTGTCGCCAGACCATCTTTT | |||
| Standard | FeLYVE1F1 | GCCAAATGCTTCAGCCTGGT | 564 | This study. Designed based on obtained ferret sequence. |
| FeLYVE1R1 | AGCCCGAGCAGAAGTAGGAG | |||
| Standard | FePROX-1F1 | ATGCCTGACCATGACAGCAC | 286 | This study. Designed based on obtained ferret sequence. |
| FePROX-1R1 | GCTGGGAAATTATGGTTGCT | |||
| Standard | FeVEGFR3F1 | TGAGCTCTATGACATCCAGC | 482 | This study. Designed based on obtained ferret sequence. |
| FeVEGFR3R1 | CCTTTAGTACCAGGGCATGC | |||
| Standard | FeCCL21F1 | ATGGCTCAGTTACTGACTCC | 310 (smaller fragment) | This study. Designed based on obtained ferret sequence. |
| FeCCL21R1 | TACAGCCCTGGACTTGTTTC | ~500 (larger fragment) | ||
| Standard | FeGAPDHF1 | CATCGTGGAGGGCCTCATGA | 215 | This study. Designed based on obtained ferret sequence. |
| FeGAPDHR1 | ATACATTGGGGGTGGGGACAC | |||
| Real time | SYBRfPDPNF1 | GAGGATGGGCCAACTCAAGA | 79 | This study. Designed based on obtained ferret sequence. |
| SYBEfPDPNR1 | GTTGTGGTGCTCTGGCTTTCT | |||
| Real time | SYBRfLYVE1F1 | GCTTCAGCCTGGTGTTGCTT | 79 | This study. Designed based on obtained ferret sequence. |
| SYBRfLYVE1R1 | GATGTGACCAGGAGCCTTGTG | |||
| Real time | SYBRfGAPDHF | TTGCTGACAATCTTGAGGGAGTT | 81 | This study. Designed based on obtained ferret sequence. |
| SYBRfGAPDHR | CTGCTGATGCCCCCATGT | |||
| Real time | SYBRfCCL20F | TGCTCCTGGCTACTTTGATGTC | 89 | This study. Designed based on published ferret CCL20 (Qin et al., 2013) |
| SYBRfCCL20R | TGCTTGCTGCTTCTGACTTG |