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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 16;194(4):1819–1831. doi: 10.4049/jimmunol.1402495

Figure 1.

Figure 1

MHV68 stimulates a blunted innate immune response in dendritic cells. (A-D) Mouse bone marrow-derived dendritic cells (BMDCs) from C57BL/6 mice were seeded and infected with MHV68 at increasing doses between MOI 1 and 1000 (in panel D between 10 and 100), MCMV (MOI 1), and HSV-1 (MOI 3). Supernatants were harvested 24 hr post infection and used for measurement of cytokines by type I IFN bioassay (A) or ELISA (B-D). Data are presented as means of measurements from triplicate cultures +/- st.dev. (E) BMDCs were treated with MCMV (MOI 10) and MHV68 (MOI 10) for 6 h, prior to harvest of total RNA, which was subjected to analysis for specific transcripts using NanoString Technology. Data are presented as heat maps. Arrows indicate type I IFN genes, while stars indicate IFN-stimulated genes. Data are representative of three independent experiments.