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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 12;194(4):1677–1685. doi: 10.4049/jimmunol.1402172

FIGURE 1.

FIGURE 1

FLVCR is required for T cell development beyond the DP stage. (A) Flow cytometric analysis of peripheral blood leukocytes by B220 and CD3 staining (upper) and CD4 and CD8 staining (lower) of cells within the CD3+ gate. Numbers indicate the percentage of cells within the gate. (B) Absolute numbers of B220+, CD3+, CD3+CD4+, and CD3+CD8+ peripheral blood lymphocytes. Bars show the mean and s.e.m. of 3–4 mice per group. *p<0.03 **p<0.009. (C) Representative gross thymuses from Flvcr+/+, Flvcr+/−, and Flvcr−/− mice (left) with representative thymus sections from Flvcr+/+ and Flvcr−/− mice stained with hematoxylin and eosin (10x magnification) (right). (D) CD4 and CD8 flow staining of Flvcr+/+ and Flvcr−/− thymocytes. (E) Enumeration of thymocyte subsets. *p=0.05 **p=0.02. (F) Heme regulatory gene expression quantified by qRT-PCR in thymocyte subsets relative to peripheral splenic CD8+ T cells. Mean and s.e.m. from 3 mice are shown. Data are representative of 2 (C, F) or 3 (A, B, D, E) separate experiments with 3–4 mice per group.