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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 19;194(4):1916–1927. doi: 10.4049/jimmunol.1401893

FIGURE 5.

FIGURE 5

MiRNA mimics differentially modulate transcription and intracellular accumulation of cytokines. MiRNA mimics, inhibitors or control transfected cell were challenged with E. coli and cytokine mRNA levels were examined by qRT-PCR after 4 h. (A) Expression levels of TNF-α, IL-6 and IL-12p40 are shown for Mφ and DC. (B) ELISA showing intracellular cytokine expression in Mφ and DC. (C) Immunostaining shows decreased intracellular TNF-α in miRNA mimic overexpressing Mφ. Images were captured using a fluorescent microscope and a 20x objective lens. Green signals indicate intracellular TNF-α. Cells were counterstained with Hoechst nuclear dye. Scale bar, 200μm. The yellow arrows point to accumulation of TNF-α localized to the cell periphery. The data represents three different fields captured from each well from at least three independent experiments. Student's t-test was conducted to calculate p values. *p< 0.01 was considered as significant.