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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Neurosci Methods. 2014 Dec 30;241:78–84. doi: 10.1016/j.jneumeth.2014.12.015

Figure 3. Pharmacological inhibition of CSF formation by acetazolamide.

Figure 3

A: CSF outflow at baseline was 1.16 μL/min; infusion of aCSF (1.93 μL/min) yielded a measured outflow rate of 2.98 μL/min, or a calculated rate of CSF formation of 1.05 μL/min, within 10% of baseline (3 rats; 11 weeks of age). B: In two different experiments, CSF outflow at baseline was 0.95±0.23 μL/min (filled circles) or 0.96±0.03 μL/min (empty circles); infusion of acetazolamide (45 mM in aCSF; pH 9.0; 1.93 μL/min) or of mannitol (88.2 mM in aCSF; pH 9.0; 1.93 μL/min) yielded calculated rates of CSF formation 0.48±0.12 μL/min (filled circles) or of 1.12±0.17 μL/min (empty circles); based on these 2 experiments, inhibition by acetazolamide was calculated to be 57%; note that inhibition by acetazolamide was not reversible, with a rate of 0.38±0.10 μL/min observed after stopping infusion (3 rats/experiment; 10 weeks of age).