Figure 4. Subcellular compartmentalization of the GAF domain ACHM mutants of PDE6C.
Live cell imaging (left) and cryosection imaging (right panels) of the GAF domain mutant EGFP-PDE6C proteins R104W, Y323N, and P391L. EGFP-fluorescence – green, WGA-staining – red, and the overlay combined with the channel for TO-PRO3 nuclear stain (blue). Scale bar - 10 μm.