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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Bioenerg Biomembr. 2014 Dec 4;47(0):149–154. doi: 10.1007/s10863-014-9593-5

FIGURE 1. Increased age in rats lead to decreases in NADH and FADH2 mediated respiration.

FIGURE 1

Total mitochondria were isolated from young (3 month) and aged (12–15 months) male Sprague-Dawley rats (9 per age group, tissue from 3 animals pooled for final n=3 per group) and oxygen consumption assessed in a sealed, stirred, thermostated chamber equipped with a Clark-type electrode and expressed as nmol of oxygen consumed per min per mg of mitochondrial protein. Maximum NADH-linked (A) and FADH2-linked (B) respiration data were then analyzed using 2way ANOVAs following by unpaired t-tests when warranted to assess age effects on mitochondrial bioenergetics. Data are expressed as % of respiration measured in young animals, *p < 0.05.