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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Bioenerg Biomembr. 2014 Dec 4;47(0):149–154. doi: 10.1007/s10863-014-9593-5

FIGURE 2. Mitochondrial ROS production increases with age.

FIGURE 2

Aged rats had a 200% to 600% increase in ROS production mitochondria isolated from the cortex and spinal cord compared to young rats. Total mitochondria were isolated from young (3 month) and aged (12–15 months) male Sprague-Dawley rats (9 per age group, tissue from 3 animals pooled for final n=3 per group) and ROS production assessed using the indicator DCF in the presence of respiratory substrates. Control wells included FCCP to induce minimum ROS production and oligomycin to induce maximum ROS production. Wells in which mitochondria were omitted were used for background subtraction. Raw data (DCF fluorescence AU/ug) were then analyzed using a 2way ANOVA following by unpaired t-tests when warranted to assess age effects on mitochondrial basal ROS production. Data are expressed as % of respiration demonstrated in young animals, *p < 0.05.