Fig. 3.
NTS is successfully induced in GW2580 treated mice. (A) Levels of serum mouse anti-rabbit IgG antibodies were assessed by ELISA to determine if GW2580 treatment interfered with the initial immunization. (B) Rabbit anti-mouse GBM antibodies in serum were measured by ELISA to ascertain that NTS/PBS mice and NTS/GW2580 mice were exposed to comparable levels of nephrotoxic antibodies. Shown are results from one experiment (control mice, n = 5; NTS/GW2580 mice, n = 10; NTS/PBS mice, n = 10), which is representative of the two independent studies performed.