Table 2.
Kinetics of dNTP incorporation with a template containing an embedded ribonucleoside.
| Nucleotides Positions |
Template | kpol (s−1) a | Kd (μM) a | kpol/Kd (μM−1s−1) a |
|---|---|---|---|---|
|
| ||||
|
dATP Position -1 |
dA | 38.05 ± 0.26 | 1.77 ± 0.28 | 21.50 ± 3.34 |
| 21-mer | rA | 1.86 ± 0.08 | 50.74 + 4.77 | 0.04 ± 0.00 |
|
| ||||
|
dTTP Position 0 |
dA | 21.99 ± 1.07 | 1.43 ± 0.19 | 15.38 ± 1.32 |
| 22-mer | rA | 91.50 ± 5.50 | 13.70 ± 1.27 | 6.68 ± 0.22 |
|
| ||||
|
dGTP Position +1 |
dA | 34.85 ± 1.93 | 1.52 ± 0.44 | 24.62 ± 5.86 |
| 23-mer | rA | 22.21 ± 1.47 | 0.56 ± 0.15 | 42.03 ± 8.68 |
a
Values represent mean ± standard deviation from two independent experiments.