Skip to main content
. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Immunol Methods. 2014 Nov 25;416:137–145. doi: 10.1016/j.jim.2014.11.009

Figure 2.

Figure 2

Cytokine production and donor-to-donor variability is increased in the LRS cone isolated cells. T cells isolated from buffy coats or LRS cones were unstimulated (US) or stimulated with plate-bound anti-human CD3 (2 μg/ml) in the absence or presence of anti-CD28 (1 μg/ml), Fibronectin (1 μg/ml) or anti-CD44 (1 μg/ml) for 48 hours at 37 degrees. Supernatants were collected and assayed by ELISA for (A) IL-2 production and (B) IFN-γ production. The averages ± SEM of six different donors for each isolation method are shown with the outliers removed as described in the Materials and Methods.