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. 2014 Nov 29;45(2):492–500. doi: 10.1002/eji.201444613

Figure 2.

Figure 2

Comparison of inhibition of CD158b+ NK cells from KIR2DL2+ and KIR2DL3+ donors by individual peptides and double peptide combinations. 721.174 cells were incubated with the indicated peptides and used as target cells in degranulation assays for CD158b‐positive NK cells from eight KIR2DL3 homozygous donors and six KIR2DL2 homozygous donors. The individual responses of KIR2DL2 (circles) and KIR2DL3 (squares) donor NK cells to (A) VAP‐FA, (B) VAP‐RA, and (C) VAP‐DA are shown. The dashed line connects the mean of the results from the KIR2DL2 homozygous donors and the full line the KIR2DL3 donors. (D–F) Responses to pairs of peptide are shown with the final concentration of peptides in all mixes being 10 μM. The concentration of each of the peptides is indicated on each x‐axis. Data show the mean value for each individual donor, and are from one experiment performed in duplicate. p values for the differences between the donors as determined by ANOVA are indicated. For comparisons at individual peptide concentration *0.05 < p < 0.1 and **p < 0.001. Degranulation data are normalized to 721.174 with no peptide.