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. 2015 Jan 17;15:4. doi: 10.1186/1472-6831-15-4

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© Onozawa et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Biofilm formation by homotypic P. gingivalis 33277 or ECF sigma factor mutants. The strains were grown on enriched BHI broth anaerobically at 37°C in collagen type-I-coated 12-well flat-bottom microplates. After 48 h of cultivation, the organized biofilm mass was evaluated by staining with crystal violet. (a) The photographs are a representative sample of each experimental strain. (b) Biofilm formation determined by crystal violet staining and adjusted for growth (A₆₀₀ units per OD₆₆₀ unit). The data shown are mean ± SD of triplicate experiments. ***, p < 0.001, by a one-way ANOVA Test/Dunnett’s Multiple Comparison Test.