Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli strain | ||
| DH5α | General-purpose host strain for cloning | Invitrogen |
| P. gingivalis strain | ||
| 33277 | wild type | ATCC |
| KDP314 | PGN_0274::ermF ermAM, Emr | This study |
| KDP315 | PGN_0319::ermF ermAM, Emr | This study |
| KDP316 | PGN_0450::ermF ermAM, Emr | This study |
| KDP317 | PGN_0970::ermF ermAM, Emr | This study |
| KDP319 | PGN_1740::ermF ermAM, Emr | This study |
| KDP314C | KDP314/pKD828, Emr Tcr | This study |
| KDP319C | KDP319/pKD829, Emr Tcr | This study |
| E. coli plasmid | ||
| pGEM-T Easy | Apr, plasmid vector for TA cloning | Promega |
| pKD355 | Apr , contains the ermF ermAM DNA cassette between EcoRI and BamHI of pUC18 | 12 |
| pKD814 | Apr, contains the 1.0-kb PCR-amplified fragment (PGN_0450 region) in pGEM-T Easy | This study |
| pKD817 | Apr, contains the 1.5-kb PCR-amplified fragment (PGN_0274 region) in pGEM-T Easy | This study |
| pKD818 | Apr, contains the 2.0-kb PCR-amplified fragment (PGN_0319 region) in pGEM-T Easy | This study |
| pKD819 | Apr, contains the 2.0-kb PCR-amplified fragment (PGN_0970 region) in pGEM-T Easy | This study |
| pKD821 | Apr, contains the 2.0-kb PCR-amplified fragment (PGN_1740 region) in pGEM-T Easy | This study |
| pKD822 | Apr Emr, contains the ermF ermAM DNA cassette at BamHI site within PGN_0274 of pKD817 | This study |
| pKD823 | Apr Emr, contains the ermF ermAM DNA cassette at BamHI site within PGN_0319 of pKD818 | This study |
| pKD824 | Apr Emr, contains the ermF ermAM DNA cassette at BamHI site within PGN_0450 of pKD814 | This study |
| pKD825 | Apr Emr, contains the ermF ermAM DNA cassette at BglII site within PGN_0970 of pKD819 | This study |
| pKD827 | Apr Emr, contains the ermF ermAM DNA cassette at BamHI site within PGN_1740 of pKD821 | This study |
| P. gingivalis plasmid | ||
| pT-COW | Apr Tcr, E. coli-P. gingivalis shuttle plasmid | 14 |
| pKD828 | Apr Tcr, pT-COW- PGN_0274+ | This study |
| pKD829 | Apr Tcr, pT-COW- PGN_1740+ | This study |