Table 1.

Nucleotide changes between Tsk2/+ mice and 101/H or B6 mice

Nucleotide position on chr 1 (mm9)	Genotype of Tsk2/+	Genotype of B6	Genotype of 101/H	Present in Other Strains?	Potential candidate for Tsk2?	Protein or mRNA containing substitution
SNP found by RNA-Seq
44,675,490	A	T	T	No	No, outside interval	Gulp1 Intron
44,833,682*	C	T	T	No	YES	Gulp1Intron
45,378,353*	A	T	T	No	YES	COL3A1 Exon (C33S)
45,432,389	C	G	Nd	Yes	No	Col5a2 3′UTR
45,441,243	C	A	C	No	No, in 101/H	Col5a2 Intron
45,860,529	G	A	G	Yes	No	Wdr75 Intron
45,874,790	T	C	T	Yes	No	Wdr75 Intron
45,875,728	C	T	C	Yes	No	Wdr75 Exon
45,880,257	CG	AC	CG	No	No, in 101/H	WDR75 Exon
46,872,610	T	G	Nd	Yes	No	Slc39a10 Intron
46,874,711	C	T	C	Yes	No	Slc39a10 Intron
46,939,340	T	C	T	Yes	No	BC040767 Intron
46,939,624	A	G	Nd	Yes	No	BC040767 Intron
SNP found by 454 Sequencing
44,833,682*	C	T	T	No	YES	Gulp1Intron
45,378,353*	A	T	T	No	YES	COL3A1 Exon (C33S)
45,465,923	A	T	T	No	YES	Col5a2 Intron
46,124,856	A	G	A	Yes	No	Dnahc76 Intron
46,124,857	A	C	T	Yes	No	Dnahc76 Intron
46,268,651	C	T	T	No	No, outside interval	Dnahc76 Intron

All single-copy nucleotide changes found by RNA-Seq or 454 sequencing were checked for their presence in other non-fibrotic strains (http://phenome.jax.org/) or individually verified by a phototyping assay (Bunce et al., 1995) and/or resequencing to confirm the single nucleotide change. SNP that were ruled out by one of these assays are considered not to be potential candidates for Tsk2. When known, genotypes shown for 101/H are from RNA-Seq, 454 sequencing or phototyping. Nd = not determined.

^*seen in both assays.