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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 21;194(4):1545–1554. doi: 10.4049/jimmunol.1402066

Figure 1.

Figure 1

cGAS and DDX41 expression in BMMs during response to IFNα and lipidA. A, BMMs were treated with 62.5 U/ml IFNα for 2.5 h, RNA was extracted and gene expression profile was detected by Affimetrix430.2 Chip, cGas mRNA level was shown as probe intensity from microarray. B, WT or Ifnar1−/− BMMs were stimulated with 100 ng/ml lipidA for indicated time points, RNA was extracted and gene expression profile was detected by RNA sequencing (RNA-seq). cGas mRNA level was shown as FPKM (fragments per kilobase of transcript per million fragments mapped). C, Ddx41 mRNA level was shown as probe intensity from the microarray data as described in (A). D, Ddx41 mRNA level was shown as FPKM from the RNA-seq data as described in (B). E, WT and Ifnar1−/− BMMs were transfected with 1 μg/ml polyI:C or polydA:dT for 4 h, cGas mRNA level in these cells was detected by qPCR and normalized to Rpl32. **p<0.01 (Student's t-test). Data are from three independent experiments (mean ± s.e.m).