Figure 4.
FoxO3 does not regulate the contraction and secondary expansion. A–B 500 OT-I CD45.1 FoxO3−/− and 500 OT-I CD45.2 were co-injected into WT (CD45.1/2) mice one day before immunization. The mice were infected with 1×106 vaccinia-OVA or with 5×106 Actm-OVAkb−/−. In A the response was measured in the blood at different time points during priming, contraction, and memory. The mice were then rechallenged at day 43 with 5000 Lm-OVA and the response was measured in the blood 5 days later. B represents the FACS plot during memory and secondary response. Data are representative of groups of 4 to 5 mice and represents the most representative result of 2 to 3 independent experiments.