Effect of delphinidin (Del) treatment on protein expression of proliferating cell
nuclear antigen (PCNA) and keratin-1, keratin-10 and keratin-14 in flaky skin
mice. (a) Immunohistochemistry of PCNA, keratin-1, keratin-10 and keratin-14 on
skin sections of flaky mice was performed. Representative pictures are shown.
Scale bar = 20 μm. (b) Western blot analysis for protein expression of
PCNA, keratin-1, keratin-10 and keratin-14 was performed in protein lysates.
Equal loading of protein was confirmed by stripping the immunoblot and reprobing
it for β-actin. The immunoblots shown here are from a representative
experiment repeated three times with similar results.