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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Br J Dermatol. 2014 Dec 23;172(2):354–364. doi: 10.1111/bjd.13513

Fig. 5.

Fig. 5

Effect of delphinidin (Del) treatment on protein and mRNA expression of interleukin (IL)-1β and tumour necrosis factor (TNF)-α in flaky skin mice. (a, b) Western blot and immunofluorescence analyses for protein expression of IL-1β and TNF-α in the flaky mice skin sections treated with and without delphinidin were performed. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for β-actin. The immunoblots shown here are from a representative experiment repeated three times with similar results. Scale bar = 20 μm. (c) Quantitative real-time polymerase chain reaction (PCR) was performed using Ssofast™ EvaGreen® Supermix (Bio-Rad Laboratories, Hercules, CA, U.S.A.) on a 7500 Fast Real-Time PCR System (Applied BioSystems, Carlsbad, CA, U.S.A.). Gene expression was determined and normalized using a value derived from the housekeeping gene GAPDH. Data shown here are relative mRNA expression ± SEM. Significant differences were determined as *P < 0.05 and **P < 0.01 vs. control.