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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Br J Dermatol. 2014 Dec 23;172(2):354–364. doi: 10.1111/bjd.13513

Fig. 6.

Fig. 6

Effect of delphinidin (Del) treatment on mRNA expression of inflammatory cytokines and activator protein (AP)-1 factor proteins in flaky skin mice. (a) Quantitative real-time polymerase chain reaction (PCR) was performed using Ssofast™ EvaGreen® Supermix (Bio-Rad Laboratories, Hercules, CA, U.S.A.) on a 7500 Fast Real-Time PCR System (Applied BioSystems, Carlsbad, CA, U.S.A.) to determine the effect of delphinidin on inflammatory cytokines. Gene expression was determined and normalized using a value derived from the housekeeping gene GAPDH. Data shown here are relative mRNA expression ± SEM. Significant differences were determined as *P < 0.05 and **P < 0.01 vs. control. (b) Western blot analysis for JunB, JunD, Fra-1 and Fra-2 protein expression was performed. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for lamin. The immunoblots shown here are from a representative experiment repeated three times with similar results. IL, interleukin; TGF-β, transforming growth factor-β.