Table 1.
Protein phosphorylation after flagellin stimulationa)
| Protein phosphorylated | Estimated fold change, | |
|---|---|---|
| Δf = F (WT, Myd88) – F (TLR5) |
||
| WT | Myd88 deficient | |
| PKCd | 0.70 | 0.92 |
| Tyro10 (DDR2) | 0.66 | 0.77 |
| Kit | 1.12 | 0.74 |
| CASP1 | 2.46 | 1.78 |
| IRS1 | 1.13 | 0.96 |
| GSK3b | 2.50 | 3.74 |
| TEK (TIE2) | 1.40 | 1.20 |
| Syk | 5.51 | 5.28 |
a)
DCs were isolated from WT, TLR5-deficient, and MyD88-deficient mice and stimulated with flagellin (5 μg/mL) for 15 min. Protein phosphorylation was determined in cell lysates with a Kinex antibody microarray, containing a panel of phospho-specific antibodies to provide a broad overview of intracellular phosphorylation. Estimated fold change (Δf) is calculated by subtracting the fold change values observed in TLR5-deficient samples (control, F-TLR5) from WT (F-Wt) and MyD88-deficient (F-Myd88) samples. Fold change (F) is the Z ratio of treated over untreated samples.