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. 2014 Dec 2;5(6):e01874-14. doi: 10.1128/mBio.01874-14

FIG 8 .

FIG 8 

Increased β-glucan exposure on the surface of killed C. albicans cells and live yeast cells from the Δmnt1/2 O-mannan mutant. The Dectin-1-Fc reporter was incubated with yeast (A) or hyphae (B) of live CAI4 (NGY152) cells (i), UV-killed (UVK) CAI4 cells (ii), heat-killed (HK) CAI4 cells (iii), or live Δmnt1/2 mutant (NGY337) cells (iv), to indicate exposure of β-glucan on the cell surface. Shown are representative images of DIC (upper panels) and Dectin-1-Fc (lower panels). The scale is indicated by the bar. (C) FACS determination of β-glucan exposure on the cell surface of wild-type SC5314 or Δmnt1/2 mutant yeast cells detected by Fc-Dectin-1. A representative histogram shows mean fluorescence intensities (black, isotype control; blue, SC5314; red, Δmnt1/2 mutant). (D) Combined data from triplicate experiments comparing SC5314 with Δmnt1/2 cells. **, P ≤ 0.01.