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. 2014 Dec 2;5(6):e02021-14. doi: 10.1128/mBio.02021-14

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Copyright © 2014 Vinayak et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 4 — Localization and expression of the T. gondii SUN protein. (A) Fluorescence microscopy of the C-terminally HA-tagged SUN protein using anti-HA (green) and IMC1 (red [to outline parasite cells]) antibodies shows its nucleolar localization. Nuclei are stained with DAPI (4′,6-diamidino-2-phenylindole) (blue). (B) SUN protein is associated with the granular compartment (outer) of the nucleolus. Fibrillarin antibody staining of the dense fibrillar compartment is shown in red, and the HA-tagged SUN protein (green) forms a ring-shaped structure around the fibrillarin. (C) Western blot of parasite pellets showing expression of HA₃-tagged SUN protein using anti-HA antibody. The TATi ΔKu80 parental line showing no expression is included as a control. The lower panel shows the loading control using anti-α-tubulin antibody.